US Ebola patient Thomas Eric Duncan has died four days after being admitted to hospital in Dallas.
Mr Duncan arrived in Dallas from Liberia two weeks ago and was admitted to Texas Presbyterian Hospital at the weekend.
Texas state health officials said they are monitoring ten people who had close contact with Mr Duncan and 38 others who came into contact with that group to see if anyone had developed signs of infection.
So far, no one has shown any symptoms, health officials said.
Mr Duncan was in contact with a known Ebola patient before he left Liberia last month.
He did not have a fever when he left the country on 19 September, but began to feel sick on 24 September after arriving in Texas.
He was initially sent home when he first sought medical care, leaving a four-day span when he was sick and contagious while in contact with others.
Meanwhile, Spanish health officials have said the nurse diagnosed with Ebola may have contracted the virus after touching her face with a glove as she removed her protective suit after treating an infected patient.
They said Teresa Romero entered the room of Spanish missionary Manual Garcia Viejo twice - once to clean him and the second time after he died on 25 September, just days after being repatriated from Sierra Leone.
Dr German Ramirez at Madrid's La Paz-Carlos III hospital said Ms Romero told him she remembers she touched her face with her gloves as she removed her protective suit after leaving the quarantine room at the hospital where the priest was being treated.
"She thinks she remembers that it was during the first time she entered the room but we should continue to look into it," he told reporters gathered outside the hospital.
"It seems like it was the gloves. The gloves touched the face," Dr Ramirez said, adding the infected nurse had authorised him to speak to the media.
"It is possible that this was not a mistake as such. It could simply be an accident and logically, probably, she could not remember at the beginning because of the state of her health."
Ms Romero had said in an interview published in El Mundo today that she had "no idea" how she could have contracted the virus.
Six people have been admitted to hospital in Madrid since Ms Romero was diagnosed with the disease on Monday in the first case of transmission of the disease outside of Africa.
In the latest case, a nurse was quarantined at La Paz-Carlos III hospital this morning as a precaution, a spokesman for the hospital said.
Another nurse was admitted to the hospital with a fever last night and put in quarantine for monitoring, he added, confirming Spanish media reports.
Three other people had been placed in quarantine at the hospital for monitoring since Ms Romero was diagnosed.
Among the six people hospitalised are the woman's husband, who is considered "high risk".
Officials said they were monitoring 52 other people, mostly health staff who had been in contact with the infected nurse, as a precaution.
Health Minister Ana Mato said that there were no signs that any of the woman's colleagues had also become infected.
"At the moment we have no evidence that any of them have any symptoms," she said as she arrived in parliament.
Meanwhile, a Spanish court has ordered that the dog owned by Ms Romero - Excalibur - be put down.
Meanwhile, travelers arriving in the United States from Liberia, Sierra Leone and Guinea will face mandatory screening measures for Ebola as soon as this weekend.
CNN is reporting that the screening could be extended to other countries struggling with the outbreak.
In addition to screening measures travelers must undergo before leaving affected countries, they will now be required to answer questions and have their temperature taken once they arrive in the US.
Britain is to send 750 troops to Sierra Leone to help build an Ebola treatment centre following a meeting of the government's emergency response committee.
Elsewhere, an international medical official with the UN Mission in Liberia has tested positive for the virus and is receiving treatment.
The official, who was not identified, is the second member of the mission to contract Ebola. The first died on 25 September.
The world's worst outbreak of Ebola has killed more than 3,400 people, predominantly in Liberia, Guinea and Sierra Leone.
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